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Methods in Molecular Biology

MicroRNA Detection and Target Identification

Methods and Protocols

Editors: Dalmay, Tamas (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6866-4
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6864-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8322-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols  guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Improved Denaturation of Small RNA Duplexes and Its Application for Northern Blotting

    Pages 1-6

    Harris, C. Jake (et al.)

  • High-Throughput RT-qPCR for the Analysis of Circulating MicroRNAs

    Pages 7-19

    Tan, Geok Wee (et al.)

  • Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum

    Pages 21-44

    Blondal, Thorarinn (et al.)

  • Small RNA Profiling by Next-Generation Sequencing Using High-Definition Adapters

    Pages 45-57

    Billmeier, Martina (et al.)

  • Surface Acoustic Wave Lysis and Ion-Exchange Membrane Quantification of Exosomal MicroRNA

    Pages 59-70

    Richards, Katherine E. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6866-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6864-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8322-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
MicroRNA Detection and Target Identification
Book Subtitle
Methods and Protocols
Editors
  • Tamas Dalmay
Series Title
Methods in Molecular Biology
Series Volume
1580
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6866-4
DOI
10.1007/978-1-4939-6866-4
Hardcover ISBN
978-1-4939-6864-0
Softcover ISBN
978-1-4939-8322-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 304
Number of Illustrations
9 b/w illustrations, 75 illustrations in colour
Topics