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Methods in Molecular Biology

Synthetic Antibodies

Methods and Protocols

Editors: Tiller, Thomas (Ed.)

  • Includes cutting-edge methods and protocols for the study of synthetic antibodies
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6857-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6855-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.

Table of contents (27 chapters)

  • Antibody Mimetics, Peptides, and Peptidomimetics

    Zhang, Xiaoying (et al.)

    Pages 3-13

  • Construction of a scFv Library with Synthetic, Non-combinatorial CDR Diversity

    Bai, Xuelian (et al.)

    Pages 15-29

  • Enzymatic Assembly for scFv Library Construction

    Kato, Mieko (et al.)

    Pages 31-44

  • Directed Evolution of Protein Thermal Stability Using Yeast Surface Display

    Traxlmayr, Michael W. (et al.)

    Pages 45-65

  • Whole Cell Panning with Phage Display

    Stark, Yvonne (et al.)

    Pages 67-91

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6857-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6855-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic Antibodies
Book Subtitle
Methods and Protocols
Editors
  • Thomas Tiller
Series Title
Methods in Molecular Biology
Series Volume
1575
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6857-2
DOI
10.1007/978-1-4939-6857-2
Hardcover ISBN
978-1-4939-6855-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 420
Number of Illustrations and Tables
28 b/w illustrations, 74 illustrations in colour
Topics