Synthetic Antibodies

1. Front Matter

   Pages i-xiv

   PDF

2. Amino Acid-Based Synthetic Antibodies

   1. Front Matter

      Pages 1-1

      PDF

   2. Antibody Mimetics, Peptides, and Peptidomimetics

      • Xiaoying Zhang, Thirumalai Diraviyam

      Pages 3-13

   3. Construction of a scFv Library with Synthetic, Non-combinatorial CDR Diversity

      • Xuelian Bai, Hyunbo Shim

      Pages 15-29

   4. Enzymatic Assembly for scFv Library Construction

      • Mieko Kato, Yoshiro Hanyu

      Pages 31-44

   5. Directed Evolution of Protein Thermal Stability Using Yeast Surface Display

      • Michael W. Traxlmayr, Eric V. Shusta

      Pages 45-65

   6. Whole Cell Panning with Phage Display

      • Yvonne Stark, Sophie Venet, Annika Schmid

      Pages 67-91

   7. Generating Conformation and Complex-Specific Synthetic Antibodies

      • Marcin Paduch, Anthony A. Kossiakoff

      Pages 93-119

   8. High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast

      • Andrea Sterner, Carolin Zehetmeier

      Pages 121-143

   9. Utilization of Selenocysteine for Site-Specific Antibody Conjugation

      • Xiuling Li, Christoph Rader

      Pages 145-164

   10. Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions

       • Emilie Renaud, Pierre Martineau, Laurence Guglielmi

       Pages 165-174

   11. Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis

       • Helena Persson, Charlotta Preger, Edyta Marcon, Johan Lengqvist, Susanne Gräslund

       Pages 175-187

   12. Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules

       • Neeraj Kohli, Melissa L. Geddie

       Pages 189-196

   13. Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells

       • Kai Zhang, Stephen J. Demarest, Xiufeng Wu, Jonathan R. Fitchett

       Pages 197-213

   14. A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells

       • Hongkai Zhang, Richard A. Lerner

       Pages 215-222

   15. A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins

       • Wouter P. R. Verdurmen, Marigona Mazlami, Andreas Plückthun

       Pages 223-236

   16. Data-Driven Antibody Engineering Using Genedata Biologics™

       • Maria Wendt, Guido Cappuccilli

       Pages 237-250

3. Nucleotide-Based Synthetic Antibodies: Aptamers

   1. Front Matter

      Pages 251-251

      PDF

   2. Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers

      • Nam Nguyen Quang, Anna Miodek, Agnes Cibiel, Frédéric Ducongé

      Pages 253-272

   3. Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules

      • Grigory S. Filonov

      Pages 273-289

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.

• Amino acid based antibodies
• Affinity reagents
• Aptamers
• Nucleotides
• Moleculary imprinted polymers
• Immunoglobulin
• Scaffold engineering

• MorphoSys AG, Discovery Alliances & Technologies, Planegg, Germany

  Thomas Tiller

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