Methods in Molecular Biology

Ethylene Signaling

Methods and Protocols

Editors: Binder, Brad M., Schaller, George E. (Eds.)

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  • Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-4939-6854-1
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About this book

This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutants and the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

 

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Gas Chromatography-Based Ethylene Measurement of Arabidopsis Seedlings

    Pages 3-10

    Yoon, Gyeong Mee (et al.)

  • Plant Ethylene Detection Using Laser-Based Photo-Acoustic Spectroscopy

    Pages 11-26

    Poel, Bram (et al.)

  • Treatment of Plants with Gaseous Ethylene and Gaseous Inhibitors of Ethylene Action

    Pages 27-39

    Tucker, Mark L. (et al.)

  • Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System

    Pages 41-46

    Song, Won-Yong (et al.)

  • Escherichia coli-Based Expression and In Vitro Activity Assay of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase and ACC Oxidase

    Pages 47-58

    Satoh, Shigeru (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-6854-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Ethylene Signaling
Book Subtitle
Methods and Protocols
Editors
  • Brad M. Binder
  • George E. Schaller
Series Title
Methods in Molecular Biology
Series Volume
1573
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6854-1
DOI
10.1007/978-1-4939-6854-1
Hardcover ISBN
978-1-4939-6852-7
Softcover ISBN
978-1-4939-8318-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 272
Number of Illustrations
22 b/w illustrations, 16 illustrations in colour
Topics