Skip to main content
SpringerLink
Log in
Book cover

Light Microscopy

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Yolanda Markaki

    1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Hartmann Harz

    1. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1563)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Modern Methods in Light Microscopy

    • Joel Ryan, Abby R. Gerhold, Vincent Boudreau, Lydia Smith, Paul S. Maddox
    Pages 1-15

  3. Advanced Fluorescence Microscopy: Sample Preparation, Fluorophores and Model Systems

    1. Front Matter

      Pages 17-17
      Download chapter PDF

    2. Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)

      • Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D. Osiewacz, Ernst H. K. Stelzer
      Pages 19-31

    3. Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy

      • Nina Jährling, Klaus Becker, Saiedeh Saghafi, Hans-Ulrich Dodt
      Pages 33-49

    4. Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis

      • Erinke van Grinsven, Chloé Prunier, Nienke Vrisekoop, Laila Ritsma
      Pages 51-71

    5. Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

      • Peter Quicke, Samuel J. Barnes, Thomas Knöpfel
      Pages 73-84

    6. FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells

      • Axel E. Kraft, Viacheslav O. Nikolaev
      Pages 85-90

    7. Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum

      • David Domozych, Anna Lietz, Molly Patten, Emily Singer, Berke Tinaz, Sandra C. Raimundo
      Pages 91-105

    8. Targeted Ablation Using Laser Nanosurgery

      • Naga Venkata Gayathri Vegesna, Paolo Ronchi, Sevi Durdu, Stefan Terjung, Rainer Pepperkok
      Pages 107-125

  4. Super and High-Resolution Optical Imaging

    1. Front Matter

      Pages 127-127
      Download chapter PDF

    2. Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells

      • Juri N. Bach, Giacomo Giacomelli, Marc Bramkamp
      Pages 129-141

    3. STED Imaging in Drosophila Brain Slices

      • Sandra Fendl, Jesús Pujol-Martí, Joel Ryan, Alexander Borst, Robert Kasper
      Pages 143-150

    4. Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells

      • Adam J. Trexler, Justin W. Taraska
      Pages 151-165

    5. Optical Coherence Microscopy

      • Rainer A. Leitgeb
      Pages 167-182

  5. Quantitative and Computational Image Analysis

    1. Front Matter

      Pages 183-183
      Download chapter PDF

    2. Designing Image Analysis Pipelines in Light Microscopy: A Rational Approach

      • Ignacio Arganda-Carreras, Philippe Andrey
      Pages 185-207

    3. Automated Analysis of Intracellular Dynamic Processes

      • Yao Yao, Ihor Smal, Ilya Grigoriev, Maud Martin, Anna Akhmanova, Erik Meijering
      Pages 209-228

    4. Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells

      • José Rino, Ana C. de Jesus, Maria Carmo-Fonseca
      Pages 229-242

    5. Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)

      • Nickolaos Nikiforos Giakoumakis, Maria Anna Rapsomaniki, Zoi Lygerou
      Pages 243-267
Back to top

Keywords

About this book

This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.

Reviews

“This well-illustrated book provides up-to-date light microscopy approaches and tool sets offered for live or fixed cell observations with a focus on system instrumentation parameters and sophisticated labeling and detection methods. … authors hope that this book will prove to be a robust teaching guide for light microscopy practical courses. I echo the authors’ opinion. … an excellent book which may serve as a user manual on current light microscopy approaches and toolsets for live or fixed cell observations.” (Omer Iqbal, Doody's Book Reviews, July, 2017)

Editors and Affiliations

  • Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, USA

    Yolanda Markaki

  • Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

Bibliographic Information

  • Book Title: Light Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Yolanda Markaki, Hartmann Harz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6810-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6808-4Published: 05 April 2017

  • Softcover ISBN: 978-1-4939-8305-6Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6810-7Published: 21 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 11 b/w illustrations, 62 illustrations in colour

  • Topics: Imaging / Radiology

Publish with us

Policies and ethics

Back to top

Search

Navigation