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Methods in Molecular Biology

RNA Methylation

Methods and Protocols

Editors: Lusser, Alexandra (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-4939-6807-7
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6805-3
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  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
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  • ISBN 978-1-4939-8304-9
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About this book

This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.

 

Table of contents (20 chapters)

Table of contents (20 chapters)
  • LC-MS Analysis of Methylated RNA

    Pages 3-18

    Thüring, Kathrin (et al.)

  • Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry

    Pages 19-32

    Paulines, Mellie June (et al.)

  • Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation

    Pages 33-42

    Yuan, Bi-Feng

  • Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)

    Pages 45-53

    Molinie, Benoit (et al.)

  • Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)

    Pages 55-78

    Grozhik, Anya V. (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6807-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6805-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8304-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Methylation
Book Subtitle
Methods and Protocols
Editors
  • Alexandra Lusser
Series Title
Methods in Molecular Biology
Series Volume
1562
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6807-7
DOI
10.1007/978-1-4939-6807-7
Hardcover ISBN
978-1-4939-6805-3
Softcover ISBN
978-1-4939-8304-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 304
Number of Illustrations
28 b/w illustrations, 34 illustrations in colour
Topics