- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.
- Table of contents (20 chapters)
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LC-MS Analysis of Methylated RNA
Pages 3-18
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Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry
Pages 19-32
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Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
Pages 33-42
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Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)
Pages 45-53
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Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
Pages 55-78
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Table of contents (20 chapters)
- Download Preface 1 PDF (21.5 KB)
- Download Sample pages 1 PDF (717.9 KB)
- Download Table of contents PDF (41 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- RNA Methylation
- Book Subtitle
- Methods and Protocols
- Editors
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- Alexandra Lusser
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1562
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-6807-7
- DOI
- 10.1007/978-1-4939-6807-7
- Hardcover ISBN
- 978-1-4939-6805-3
- Softcover ISBN
- 978-1-4939-8304-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 304
- Number of Illustrations
- 28 b/w illustrations, 34 illustrations in colour
- Topics
