Histochemistry of Single Molecules
Methods and Protocols
Editors: Pellicciari, Carlo, Biggiogera, Marco (Eds.)
Free Preview- Includes cutting-edge techniques for the study of single molecule histochemistry
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.
- Table of contents (26 chapters)
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Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes
Pages 3-13
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Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues
Pages 15-43
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Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree
Pages 45-53
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Histochemical Demonstration of Tripeptidyl Aminopeptidase I
Pages 55-68
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Enzyme Histochemistry for Functional Histology in Invertebrates
Pages 69-90
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Histochemistry of Single Molecules
- Book Subtitle
- Methods and Protocols
- Editors
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- Carlo Pellicciari
- Marco Biggiogera
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1560
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-6788-9
- DOI
- 10.1007/978-1-4939-6788-9
- Hardcover ISBN
- 978-1-4939-6787-2
- Softcover ISBN
- 978-1-4939-8301-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 341
- Number of Illustrations
- 11 b/w illustrations, 59 illustrations in colour
- Topics
