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Methods in Molecular Biology

Exosomes and Microvesicles

Methods and Protocols

Editors: Hill, Andrew F. (Ed.)

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  • Includes cutting-edge methods and protocols 
    Provides step-by-step detail essential for reproducible results 
    Contains key notes and implementation advice from the experts

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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6728-5
  • Digitally watermarked, DRM-free
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Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-6726-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8284-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.

 

About the authors

Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute, 
Email: a.hill@unimelb.edu.au

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Methods to Analyze EVs

    Pages 1-20

    Giebel, Bernd (et al.)

  • Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles

    Pages 21-33

    Maas, Sybren L. N. (et al.)

  • Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis

    Pages 35-42

    McNicholas, Kym (et al.)

  • Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy

    Pages 43-54

    Linares, Romain (et al.)

  • Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR

    Pages 55-70

    Bellingham, Shayne A. (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6728-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-6726-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8284-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Exosomes and Microvesicles
Book Subtitle
Methods and Protocols
Editors
  • Andrew F. Hill
Series Title
Methods in Molecular Biology
Series Volume
1545
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6728-5
DOI
10.1007/978-1-4939-6728-5
Hardcover ISBN
978-1-4939-6726-1
Softcover ISBN
978-1-4939-8284-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 228
Number of Illustrations
18 b/w illustrations, 21 illustrations in colour
Topics