Mycotoxigenic Fungi

1. Front Matter

   Pages i-xi

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2. Fungal Genera and Species of Major Significance and Their Associated Mycotoxins

   1. Front Matter

      Pages 1-1

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   2. Mycotoxins: An Underhand Food Problem

      • Antonio Moretti, Antonio F. Logrieco, Antonia Susca

      Pages 3-12

   3. Alternaria Species and Their Associated Mycotoxins

      • Virginia Elena Fernández Pinto, Andrea Patriarca

      Pages 13-32

   4. Aspergillus Species and Their Associated Mycotoxins

      • Giancarlo Perrone, Antonia Gallo

      Pages 33-49

   5. Fusarium Species and Their Associated Mycotoxins

      • Gary P. Munkvold

      Pages 51-106

   6. Penicillium Species and Their Associated Mycotoxins

      • Giancarlo Perrone, Antonia Susca

      Pages 107-119

3. Polymerase Chain Reaction (PCR)-Based Methods for Detection and Identification of Mycotoxigenic Fungi

   1. Front Matter

      Pages 121-121

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   2. Targeting Conserved Genes in Alternaria Species

      • Miguel Ángel Pavón, Inés María López-Calleja, Isabel González, Rosario Martín, Teresa García

      Pages 123-129

   3. Targeting Conserved Genes in Aspergillus Species

      • Sándor Kocsubé, János Varga

      Pages 131-140

   4. Targeting Conserved Genes in Fusarium Species

      • Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, M. Teresa González-Jaén

      Pages 141-147

   5. Targeting Conserved Genes in Penicillium Species

      • Stephen W. Peterson

      Pages 149-157

   6. Targeting Aflatoxin Biosynthetic Genes

      • Ali Y. Srour, Ahmad M. Fakhoury, Robert L. Brown

      Pages 159-171

   7. Targeting Trichothecene Biosynthetic Genes

      • Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, Cees Waalwijk

      Pages 173-189

   8. Targeting Ochratoxin Biosynthetic Genes

      • Antonia Gallo, Giancarlo Perrone

      Pages 191-200

   9. Targeting Fumonisin Biosynthetic Genes

      • Robert H. Proctor, Martha M. Vaughan

      Pages 201-214

   10. Targeting Other Mycotoxin Biosynthetic Genes

       • María J. Andrade, Mar Rodríguez, Juan J. Córdoba, Alicia Rodríguez

       Pages 215-235

   11. Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi

       • Paula Cristina Azevedo Rodrigues, Jéssica Gil-Serna, M. Teresa González-Jaén

       Pages 237-247

   12. Evaluating Fumonisin Gene Expression in Fusarium verticillioides

       • Valeria Scala, Ivan Visentin, Francesca Cardinale

       Pages 249-257

4. Polymerase Chain Reaction (PCR)-Based Methods for Multiplex Detection of Mycotoxigenic Fungi

   1. Front Matter

      Pages 259-259

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This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.

• Mycotoxins
• Toxic fungal metabolites
• Polymerase Chain Reaction
• Crop plant contamination
• Agro-ecology
• Food and feed
• Fungal secondary metabolism

• Institute of Sciences of Food Production National Research Council, Bari, Italy

  Antonio Moretti, Antonia Susca

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