Methods in Molecular Biology

Hepatitis B Virus

Methods and Protocols

Editors: Guo, Haitao, Cuconati, Andrea (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-6700-1
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6698-1
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  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
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  • ISBN 978-1-4939-8277-6
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About this book

This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.

 

Table of contents (24 chapters)

Table of contents (24 chapters)
  • NTCP-Reconstituted In Vitro HBV Infection System

    Pages 1-14

    Sun, Yinyan (et al.)

  • Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells, and Primary Human Hepatocytes

    Pages 15-25

    Ni, Yi (et al.)

  • Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells

    Pages 27-36

    König, Alexander (et al.)

  • Intracytoplasmic Transport of Hepatitis B Virus Capsids

    Pages 37-51

    Osseman, Quentin (et al.)

  • A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein

    Pages 53-58

    Yang, Ching-Chun (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6700-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6698-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8277-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hepatitis B Virus
Book Subtitle
Methods and Protocols
Editors
  • Haitao Guo
  • Andrea Cuconati
Series Title
Methods in Molecular Biology
Series Volume
1540
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6700-1
DOI
10.1007/978-1-4939-6700-1
Hardcover ISBN
978-1-4939-6698-1
Softcover ISBN
978-1-4939-8277-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 297
Number of Illustrations
26 b/w illustrations, 31 illustrations in colour
Topics