Oral Biology
Molecular Techniques and Applications
Editors: Seymour, Gregory J., Cullinan, Mary P., Heng, Nicholas C.K. (Eds.)
Free Preview- Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
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This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.
- Table of contents (29 chapters)
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Salivary Diagnostics Using Purified Nucleic Acids
Pages 3-15
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RNA Sequencing Analysis of Salivary Extracellular RNA
Pages 17-36
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Qualitative and Quantitative Proteome Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry
Pages 37-60
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Antioxidant Micronutrients and Oxidative Stress Biomarkers
Pages 61-77
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NMR-Based Metabolomics of Oral Biofluids
Pages 79-105
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Table of contents (29 chapters)
- Download Preface 1 PDF (96.3 KB)
- Download Sample pages 1 PDF (339.7 KB)
- Download Table of contents PDF (1.5 MB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Oral Biology
- Book Subtitle
- Molecular Techniques and Applications
- Editors
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- Gregory J. Seymour
- Mary P. Cullinan
- Nicholas C.K. Heng
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1537
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-6685-1
- DOI
- 10.1007/978-1-4939-6685-1
- Hardcover ISBN
- 978-1-4939-6683-7
- Softcover ISBN
- 978-1-4939-8272-1
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIV, 503
- Number of Illustrations
- 38 b/w illustrations, 54 illustrations in colour
- Topics
