Methods in Molecular Biology

Bacterial Pathogenesis

Methods and Protocols

Editors: Nordenfelt, Pontus, Collin, Mattias (Eds.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains keynotes and implementation advice from the experts

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eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-6673-8
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Hardcover $179.99
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Softcover $159.00
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About this book

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors

    Pages 3-15

    Lood, Rolf (et al.)

  • Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics

    Pages 17-24

    Karlsson, Christofer (et al.)

  • Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein–Metabolite Interactions in Bacteria

    Pages 25-41

    Orr, Mona W. (et al.)

  • Identifying Bacterial Immune Evasion Proteins Using Phage Display

    Pages 43-61

    Fevre, Cindy (et al.)

  • Competition for Iron Between Host and Pathogen: A Structural Case Study on Helicobacter pylori

    Pages 65-75

    Xia, Wei

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-6673-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $159.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Pathogenesis
Book Subtitle
Methods and Protocols
Editors
  • Pontus Nordenfelt
  • Mattias Collin
Series Title
Methods in Molecular Biology
Series Volume
1535
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6673-8
DOI
10.1007/978-1-4939-6673-8
Hardcover ISBN
978-1-4939-6671-4
Softcover ISBN
978-1-4939-8268-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 357
Number of Illustrations
23 b/w illustrations, 48 illustrations in colour
Topics