Oncogene-Induced Senescence

1. Front Matter

   Pages i-xi

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2. The Immortal Senescence

   • Anna Bianchi-Smiraglia, Brittany C. Lipchick, Mikhail A. Nikiforov

   Pages 1-15

3. Senescence Phenotypes Induced by Ras in Primary Cells

   • Lena Lau, Gregory David

   Pages 17-30

4. Cellular Model of p21-Induced Senescence

   • Michael Shtutman, Bey-Dih Chang, Gary P. Schools, Eugenia V. Broude

   Pages 31-39

5. Detecting Markers of Therapy-Induced Senescence in Cancer Cells

   • Dorothy N. Y. Fan, Clemens A. Schmitt

   Pages 41-52

6. Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence

   • Maria V. Guijarro, Amancio Carnero

   Pages 53-68

7. Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence

   • Priyanka L. Patel, Utz Herbig

   Pages 69-78

8. RT-qPCR Detection of Senescence-Associated Circular RNAs

   • Amaresh C. Panda, Kotb Abdelmohsen, Myriam Gorospe

   Pages 79-87

9. Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy

   • Masako Narita, Masashi Narita

   Pages 89-98

10. Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis

    • Priya Hari, Juan Carlos Acosta

    Pages 99-109

11. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

    • Konstantinos Evangelou, Vassilis G. Gorgoulis

    Pages 111-119

12. Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts

    • Katerina I. Leonova, David A. Scott

    Pages 121-125

13. Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence

    • Hengrui Zhu, Linh Le, Hsin-Yao Tang, David W. Speicher, Rugang Zhang

    Pages 127-137

14. Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence

    • Rabii Ameziane-El-Hassani, Corinne Dupuy

    Pages 139-145

15. Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach

    • Mohammad Althubiti, Salvador Macip

    Pages 147-153

16. Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence

    • Claudia Gey, Karsten Seeger

    Pages 155-163

17. Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence

    • Mikhail A. Nikiforov, Donna S. Shewach

    Pages 165-173

18. Senescence-Like Phenotypes in Human Nevi

    • Andrew Joselow, Darren Lynn, Tamara Terzian, Neil F. Box

    Pages 175-184

19. Detection of Oncogene-Induced Senescence In Vivo

    • Kwan-Hyuck Baek, Sandra Ryeom

    Pages 185-198

20. Detection of Senescence Markers During Mammalian Embryonic Development

    • Mekayla Storer, William M. Keyes

    Pages 199-210

This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.

• Senescent cells
• Phenotypes
• Cellular processes
• Oncology
• Oncoproteins

• Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, USA

  Mikhail A. Nikiforov

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