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Methods in Molecular Biology

Epstein Barr Virus

Methods and Protocols

Editors: Minarovits, Janos, Niller, Hans Helmut (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-6655-4
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
Softcover $119.00
price for USA in USD
About this book

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Reviews

“This is an excellent book with a good number of color illustrations providing recent immunological and cytogenetic methods and protocols aimed at finding easy solutions to unresolved problems in the field of Epstein Barr virus. … The book is appropriate for students and scientists interested in Epstein Barr Virus (EBV) and EBV diagnostics. … The book has high quality color illustrations to promote easy understanding of this complex subject.” (Omer Iqbal, Doody's Book Reviews, April, 2017)


Table of contents (19 chapters)

Table of contents (19 chapters)
  • Current Trends and Alternative Scenarios in EBV Research

    Pages 1-32

    Minarovits, Janos (et al.)

  • Epstein-Barr Virus: Clinical Diagnostics

    Pages 33-55

    Niller, Hans-Helmut (et al.)

  • Establishment of EBV-Infected Lymphoblastoid Cell Lines

    Pages 57-64

    Nagy, Noemi

  • Generation and Infection of Organotypic Cultures with Epstein–Barr Virus

    Pages 65-78

    Temple, Rachel M. (et al.)

  • Affinity Purification–Mass Spectroscopy Methods for Identifying Epstein–Barr Virus–Host Interactions

    Pages 79-92

    Georges, Anna A. (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-6655-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
Softcover $119.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Epstein Barr Virus
Book Subtitle
Methods and Protocols
Editors
  • Janos Minarovits
  • Hans Helmut Niller
Series Title
Methods in Molecular Biology
Series Volume
1532
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6655-4
DOI
10.1007/978-1-4939-6655-4
Hardcover ISBN
978-1-4939-6653-0
Softcover ISBN
978-1-4939-8262-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 269
Number of Illustrations
1 b/w illustrations, 21 illustrations in colour
Topics