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Antibiotics

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Peter Sass

    1. Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1520)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Production and Design

    1. Front Matter

      Pages 1-1
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    2. Antibiotics: Precious Goods in Changing Times

      • Peter Sass
      Pages 3-22

    3. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters

      • Martina Adamek, Marius Spohn, Evi Stegmann, Nadine Ziemert
      Pages 23-47

    4. Production of Antimicrobial Compounds by Fermentation

      • Henrik Harms, Gabriele M. König, Till F. Schäberle
      Pages 49-61

    5. Structure Elucidation of Antibiotics by NMR Spectroscopy

      • Georgios Daletos, Elena Ancheeva, Raha S. Orfali, Victor Wray, Peter Proksch
      Pages 63-83

    6. Computer-Aided Drug Design Methods

      • Wenbo Yu, Alexander D. MacKerell Jr.
      Pages 85-106

    7. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents

      • Alexander Zipperer, Dorothee Kretschmer
      Pages 107-118

  3. Mode of Action

    1. Front Matter

      Pages 119-119
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    2. Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds

      • Carolin Martina Kobras, Thorsten Mascher, Susanne Gebhard
      Pages 121-131

    3. Determination of Bacterial Membrane Impairment by Antimicrobial Agents

      • Miriam Wilmes, Hans-Georg Sahl
      Pages 133-143

    4. Mass-Sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes

      • Sebastian G. Hoß, Gerd Bendas
      Pages 145-157

    5. Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy

      • Kathi Scheinpflug, Oxana Krylova, Henrik Strahl
      Pages 159-174

    6. In Vitro Assays to Identify Antibiotics Targeting DNA Metabolism

      • Allan H. Pang, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov
      Pages 175-200

    7. Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors

      • Yu Chen, Xin Liu, Nancy Wu, Carol A. Fierke
      Pages 201-225

    8. Reporter Gene-Based Screening for TPP Riboswitch Activators

      • Christina E. Lünse, Günter Mayer
      Pages 227-235

    9. Cell-Based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation

      • Federica Briani
      Pages 237-245

    10. Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen

      • Mike Gajdiss, Michael Türck, Gabriele Bierbaum
      Pages 247-259

  4. Response and Susceptibility

    1. Front Matter

      Pages 261-261
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    2. Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution

      • Shingo Suzuki, Takaaki Horinouchi, Chikara Furusawa
      Pages 263-279
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Keywords

About this book

This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies.  Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research.

Editors and Affiliations

  • Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

    Peter Sass

Bibliographic Information

  • Book Title: Antibiotics

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Sass

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6634-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6632-5Published: 22 November 2016

  • Softcover ISBN: 978-1-4939-8255-4Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6634-9Published: 22 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 358

  • Number of Illustrations: 57 b/w illustrations, 21 illustrations in colour

  • Topics: Microbiology

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