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Hypertension

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Rhian M. Touyz

    1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Ernesto L. Schiffrin

    1. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1527)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Large-Scale Transcriptome Analysis

    • David Weaver, Kathirvel Gopalakrishnan, Bina Joe
    Pages 1-26

  3. Methods to Assess Genetic Risk Prediction

    • Christin Schulz, Sandosh Padmanabhan
    Pages 27-40

  4. Microarray Analysis of Hypertension

    • Henry L. Keen, Curt D. Sigmund
    Pages 41-52

  5. Tissue Proteomics in Vascular Disease

    • Amaya Albalat, William Mullen, Holger Husi, Harald Mischak
    Pages 53-60

  6. Urine Metabolomics in Hypertension Research

    • Sofia Tsiropoulou, Martin McBride, Sandosh Padmanabhan
    Pages 61-68

  7. Systems Biology Approach in Hypertension Research

    • Christian Delles, Holger Husi
    Pages 69-79

  8. Measurement of Angiotensin Peptides: HPLC-RIA

    • K. Bridget Brosnihan, Mark C. Chappell
    Pages 81-99

  9. Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)

    • Fengxia Xiao, Kevin D. Burns
    Pages 101-115

  10. Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate

    • Srinivas Sriramula, Kim Brint Pedersen, Huijing Xia, Eric Lazartigues
    Pages 117-126

  11. Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-Chip/Mass Spectrometry, and Functional Assays

    • Walmor C. De Mello, Yamil Gerena
    Pages 127-137

  12. Analysis of the Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes

    • Scott M. MacKenzie, Eleanor Davies, Samantha Alvarez-Madrazo
    Pages 139-150

  13. Dopaminergic Immunofluorescence Studies in Kidney Tissue

    • J. J. Gildea, R. E. Van Sciver, H. E. McGrath, B. A. Kemp, P. A. Jose, R. M. Carey et al.
    Pages 151-161

  14. Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (NPs)

    • Adolfo J. de Bold, Mercedes L. de Bold
    Pages 163-176

  15. Intracellular Free Calcium Measurement Using Confocal Imaging

    • Ghassan Bkaily, Johny Al-Khoury, Yanick Simon, Danielle Jacques
    Pages 177-187

  16. Measuring T-Type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells

    • Ivana Y. Kuo, Caryl E. Hill
    Pages 189-200

  17. In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs

    • Katherine J. Elliott, Satoru Eguchi
    Pages 201-211

  18. In Vitro and In Vivo Approaches to Assess Rho Kinase Activity

    • Vincent Sauzeau, Gervaise Loirand
    Pages 213-218

  19. NADPH Oxidases and Measurement of Reactive Oxygen Species

    • Angelica Amanso, Alicia N. Lyle, Kathy K. Griendling
    Pages 219-232

  20. Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation

    • Denise C. Fernandes, Renata C. Gonçalves, Francisco R. M. Laurindo
    Pages 233-249
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Keywords

About this book

This volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on genetics and mmics of hypertension; the Renin-Angiotensin-Aldosterone System; vasoactive agents and hypertension; signal transduction and reactive oxygen species; novel cell models and approaches to study molecular mechanisms of hypertension; vascular physiology; and new approaches to manipulate mouse models to study molecular mechanisms of hypertension. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

  

Authoritative and cutting-edge, Hypertension: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  • Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

Bibliographic Information

  • Book Title: Hypertension

  • Book Subtitle: Methods and Protocols

  • Editors: Rhian M. Touyz, Ernesto L. Schiffrin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6625-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6623-3Published: 24 January 2017

  • Softcover ISBN: 978-1-4939-8253-0Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6625-7Published: 23 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 437

  • Number of Illustrations: 38 b/w illustrations, 40 illustrations in colour

  • Topics: Molecular Medicine

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