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Liposomes

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1522)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Preparation of DRV Liposomes

    • Sophia G. Antimisiaris
    Pages 23-47
  3. Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID

    • Sophia Hatziantoniou, Costas Demetzos
    Pages 49-54
  4. Liposome Formulations of Hydrophobic Drugs

    • Reto A. Schwendener, Herbert Schott
    Pages 73-82
  5. A “Dock and Lock” Approach to Preparation of Targeted Liposomes

    • Marina V. Backer, Joseph M. Backer
    Pages 83-92
  6. Coupling of Ligands to the Liposome Surface by Click Chemistry

    • Maria Vittoria Spanedda, Marcella De Giorgi, Fatouma Saïd Hassane, Francis Schuber, Line Bourel-Bonnet, Benoît Frisch
    Pages 93-106
  7. Environmental Scanning Electron Microscope Imaging of Vesicle Systems

    • Yvonne Perrie, Habib Ali, Daniel J Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, Anil Vangala
    Pages 131-143
  8. Liposome Biodistribution via Europium Complexes

    • Nathalie Mignet, Daniel Scherman
    Pages 145-154
  9. Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes

    • Suna Erdogan, Vladimir P. Torchilin
    Pages 179-192
  10. Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes

    • Nicolas Bertrand, Pierre Simard, Jean-Christophe Leroux
    Pages 193-207
  11. Long-Circulating, pH-Sensitive Liposomes

    • Denitsa Momekova, Stanislav Rangelov, Nikolay Lambov
    Pages 209-226
  12. Anionic pH-Sensitive Lipoplexes

    • Nathalie Mignet, Daniel Scherman
    Pages 227-236

About this book

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry.

Editors and Affiliations

  • School of Pharmacy – Boston, MCPHS University, Boston, USA

    Gerard G.M. D'Souza

Bibliographic Information

  • Book Title: Liposomes

  • Book Subtitle: Methods and Protocols

  • Editors: Gerard G.M. D'Souza

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6591-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6589-2Published: 11 November 2016

  • Softcover ISBN: 978-1-4939-8244-8Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6591-5Published: 09 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 285

  • Number of Illustrations: 46 b/w illustrations, 13 illustrations in colour

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access