Methods in Molecular Biology

Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Editors: Wajapeyee, Narendra, Gupta, Romi (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

            This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators

    Misra, Ashish (et al.)

    Pages 1-12

  • Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins

    Beyer, Sophie (et al.)

    Pages 13-21

  • Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos

    Gentsch, George E. (et al.)

    Pages 23-42

  • Systematic Discovery of Chromatin-Bound Protein Complexes from ChIP-seq Datasets

    Giannopoulou, Eugenia (et al.)

    Pages 43-58

  • Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos

    Doganli, Canan (et al.)

    Pages 59-66

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6518-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6516-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-8223-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
Editors
  • Narendra Wajapeyee
  • Romi Gupta
Series Title
Methods in Molecular Biology
Series Volume
1507
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6518-2
DOI
10.1007/978-1-4939-6518-2
Hardcover ISBN
978-1-4939-6516-8
Softcover ISBN
978-1-4939-8223-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 278
Number of Illustrations
20 b/w illustrations, 29 illustrations in colour
Topics