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Methods in Molecular Biology

High-Throughput Glycomics and Glycoproteomics

Methods and Protocols

Editors: Lauc, Gordan, Wuhrer, Manfred (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-6493-2
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Hardcover $159.99
price for USA in USD
Softcover $139.00
price for USA in USD
About this book

This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Ubiquitous Importance of Protein Glycosylation

    Pages 1-12

    Krištić, Jasminka (et al.)

  • Laboratory Experimental Design for a Glycomic Study

    Pages 13-19

    Ugrina, Ivo (et al.)

  • High-Throughput Analysis of the IgG N-Glycome by UPLC-FLR

    Pages 21-29

    Pučić-Baković, Maja

  • High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS

    Pages 31-47

    Falck, David (et al.)

  • Sialic Acid Derivatization for the Rapid Subclass- and Sialic Acid Linkage-Specific MALDI-TOF-MS Analysis of IgG Fc-Glycopeptides

    Pages 49-62

    Haan, Noortje (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-6493-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
High-Throughput Glycomics and Glycoproteomics
Book Subtitle
Methods and Protocols
Editors
  • Gordan Lauc
  • Manfred Wuhrer
Series Title
Methods in Molecular Biology
Series Volume
1503
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-6493-2
DOI
10.1007/978-1-4939-6493-2
Hardcover ISBN
978-1-4939-6491-8
Softcover ISBN
978-1-4939-8217-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 274
Number of Illustrations
9 b/w illustrations, 42 illustrations in colour
Topics