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3D DNA Nanostructure

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Yonggang Ke

    1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Pengfei Wang

    1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of 3D DNA nanostructure
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1500)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. DNA/RNA Structure Design

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Designed 3D DNA Crystals

      • Nadrian C. Seeman, Ruojie Sha, Jens Birktoft, Jianping Zheng, Wenyan Liu, Tong Wang et al.
      Pages 3-10

    3. Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs

      • Cheng Tian, Chuan Zhang
      Pages 11-26

    4. Design of Wireframe DNA Nanostructures—DNA Gridiron

      • Dongran Han
      Pages 27-40

    5. Complex DNA Brick Assembly

      • Luvena L. Ong, Yonggang Ke
      Pages 41-49

    6. Computer-Aided Design of RNA Origami Structures

      • Steffen L. Sparvath, Cody W. Geary, Ebbe S. Andersen
      Pages 51-80

    7. Assembling RNA Nanoparticles

      • Shou-Jun Xiao
      Pages 81-96

  3. Applications of DNA Structures

    1. Front Matter

      Pages 97-97
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    2. DNA Functionalization of Nanoparticles

      • Fang Lu, Oleg Gang
      Pages 99-107

    3. Purification Techniques for Three-Dimensional DNA Nanostructures

      • Travis A. Meyer
      Pages 109-119

    4. DNA Nanostructure as Smart Carriers for Drug Delivery

      • Xiangyuan Ouyang, Jie Chao, Shao Su, Chunhai Fan
      Pages 121-132

    5. DNA G-Quadruplex-Based Assay of Enzyme Activity

      • Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie et al.
      Pages 133-151

    6. Spatial Organization of Enzyme Cascade on a DNA Origami Nanostructure

      • Jinglin Fu, Tianran Li
      Pages 153-164

    7. Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron

      • Steven D. Perrault, William M. Shih
      Pages 165-184

    8. DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures

      • Mingjie Dai
      Pages 185-202

    9. Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins

      • John Min, William M. Shih, Gaëtan Bellot
      Pages 203-215

    10. Direct Nanofabrication Using DNA Nanostructure

      • Feng Zhou, Haitao Liu
      Pages 217-235

    11. Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds

      • Wei Sun, Jie Shen
      Pages 237-244

    12. DNA-Directed Self-Assembly of Highly Ordered and Dense Single-Walled Carbon Nanotube Arrays

      • Hareem Maune, Si-ping Han
      Pages 245-256
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Keywords

About this book

This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, 3D DNA Nanostructure: Methods and Protocols provides the most up-to-date tutorial style overviews and technical style protocols to benefit researchers in a wide variety of areas.

Editors and Affiliations

  • Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, USA

    Yonggang Ke, Pengfei Wang

Bibliographic Information

  • Book Title: 3D DNA Nanostructure

  • Book Subtitle: Methods and Protocols

  • Editors: Yonggang Ke, Pengfei Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6454-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6452-9Published: 04 November 2016

  • Softcover ISBN: 978-1-4939-8205-9Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6454-3Published: 03 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 5 b/w illustrations, 75 illustrations in colour

  • Topics: Human Genetics

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