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Methods in Molecular Biology

Genotyping

Methods and Protocols

Editors: White, Stefan J., Cantsilieris, Stuart (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-4939-6442-0
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6440-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-8201-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems

    Pages 1-15

    Krüger, Jacqueline (et al.)

  • Genotyping DNA Variants with High-Resolution Melting Analysis

    Pages 17-28

    Vossen, Rolf H. A. M.

  • High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays

    Pages 29-57

    Heissl, Angelika (et al.)

  • In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification

    Pages 59-76

    Krzywkowski, Tomasz (et al.)

  • The MassARRAY® System for Targeted SNP Genotyping

    Pages 77-94

    Ellis, Justine A. (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6442-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6440-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-8201-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Genotyping
Book Subtitle
Methods and Protocols
Editors
  • Stefan J. White
  • Stuart Cantsilieris
Series Title
Methods in Molecular Biology
Series Volume
1492
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6442-0
DOI
10.1007/978-1-4939-6442-0
Hardcover ISBN
978-1-4939-6440-6
Softcover ISBN
978-1-4939-8201-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 254
Number of Illustrations
10 b/w illustrations, 55 illustrations in colour
Topics