Genotyping
Methods and Protocols
Editors: White, Stefan J., Cantsilieris, Stuart (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
- Table of contents (17 chapters)
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Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems
Pages 1-15
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Genotyping DNA Variants with High-Resolution Melting Analysis
Pages 17-28
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High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays
Pages 29-57
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In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification
Pages 59-76
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The MassARRAY® System for Targeted SNP Genotyping
Pages 77-94
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Table of contents (17 chapters)
- Download Preface 1 PDF (22.2 KB)
- Download Sample pages 1 PDF (4 MB)
- Download Table of contents PDF (32.9 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Genotyping
- Book Subtitle
- Methods and Protocols
- Editors
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- Stefan J. White
- Stuart Cantsilieris
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1492
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-6442-0
- DOI
- 10.1007/978-1-4939-6442-0
- Hardcover ISBN
- 978-1-4939-6440-6
- Softcover ISBN
- 978-1-4939-8201-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 254
- Number of Illustrations
- 10 b/w illustrations, 55 illustrations in colour
- Topics
