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  • © 2016

Plant Synthetic Promoters

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the study of plant synthetic promoters
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1482)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Quantitative Analysis of Protein–DNA Interaction by qDPI-ELISA

    • Stefan M. Fischer, Alexander Böser, Jan P. Hirsch, Dierk Wanke
    Pages 49-66
  3. Analyzing Synthetic Promoters Using Arabidopsis Protoplasts

    • Ralf Stracke, Katharina Thiedig, Melanie Kuhlmann, Bernd Weisshaar
    Pages 67-81
  4. Selecting Hypomethylated Genomic Regions Using MRE-Seq

    • Elisabeth Wischnitzki, Kornel Burg, Maria Berenyi, Eva Maria Sehr
    Pages 83-102
  5. Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues

    • Tou Cheu Xiong, Frédéric Sanchez, Jean-François Briat, Frédéric Gaymard, Christian Dubos
    Pages 103-110
  6. Novel Synthetic Promoters from the Cestrum Yellow Leaf Curling Virus

    • Dipak Kumar Sahoo, Shayan Sarkar, Indu B. Maiti, Nrisingha Dey
    Pages 111-138
  7. Fast and Efficient Cloning of Cis-Regulatory Sequences for High-Throughput Yeast One-Hybrid Analyses of Transcription Factors

    • Zsolt Kelemen, Jonathan Przybyla-Toscano, Nicolas Tissot, Loïc Lepiniec, Christian Dubos
    Pages 139-149
  8. The Physcomitrella patens System for Transient Gene Expression Assays

    • Johanne Thévenin, Wenjia Xu, Louise Vaisman, Loïc Lepiniec, Bertrand Dubreucq, Christian Dubos
    Pages 151-161
  9. Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System

    • Konstantin Kanofsky, Mona Lehmeyer, Jutta Schulze, Reinhard Hehl
    Pages 163-174
  10. GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis

    • Anna Coll, Mandy L. Wilson, Kristina Gruden, Jean Peccoud
    Pages 219-232
  11. In Silico Expression Analysis

    • Julio Bolívar, Reinhard Hehl, Lorenz Bülow
    Pages 247-257
  12. RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes

    • Bruno Contreras-Moreira, Jaime A. Castro-Mondragon, Claire Rioualen, Carlos P. Cantalapiedra, Jacques van Helden
    Pages 279-295
  13. RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes

    • Jaime A. Castro-Mondragon, Claire Rioualen, Bruno Contreras-Moreira, Jacques van Helden
    Pages 297-322

About this book

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. 

Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Editors and Affiliations

  • Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany

    Reinhard Hehl

Bibliographic Information

  • Book Title: Plant Synthetic Promoters

  • Book Subtitle: Methods and Protocols

  • Editors: Reinhard Hehl

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6396-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6394-2Published: 25 August 2016

  • Softcover ISBN: 978-1-4939-8186-1Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6396-6Published: 24 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 325

  • Number of Illustrations: 8 b/w illustrations, 78 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access