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Wnt Signaling

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Quinn Barrett

    1. University of Texas Southwestern Medical Center, Dallas, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Lawrence Lum

    1. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of Wnt signaling
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1481)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Visualizing Wnt Palmitoylation in Single Cells

    • Xinxin Gao, Rami N. Hannoush
    Pages 1-9

  3. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction

    • Rubina Tuladhar, Nageswari Yarravarapu, Lawrence Lum
    Pages 11-16

  4. Biochemical Methods to Analyze Wnt Protein Secretion

    • Kathrin Glaeser, Michael Boutros, Julia Christina Gross
    Pages 17-28

  5. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum

    • Xinjun Zhang, Xi He
    Pages 29-38

  6. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway

    • Xiaomo Jiang, Feng Cong
    Pages 39-48

  7. A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells

    • Annette S. Flozak, Anna P. Lam, Cara J. Gottardi
    Pages 49-60

  8. Wnt-Dependent Control of Cell Polarity in Cultured Cells

    • Kristin B. Runkle, Eric S. Witze
    Pages 61-68

  9. The Use of Chick Embryos to Study Wnt Activity Gradients

    • Lisa M. Galli, Tiffany Barnes, Laura W. Burrus
    Pages 69-80

  10. Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors

    • Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, Natascia Tiso
    Pages 81-94

  11. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo

    • Jesung Moon, James F. Amatruda
    Pages 95-100

  12. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts

    • Annastasia Simone Hyde, Brian I. Hang, Ethan Lee
    Pages 101-109

  13. Delivery of the Porcupine Inhibitor WNT974 in Mice

    • Li-shu Zhang, Lawrence Lum
    Pages 111-117

  14. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis

    • Zhendong A. Zhong, Nicole J. Ethen, Bart O. Williams
    Pages 119-125

  15. Monitoring Wnt/β-Catenin Signaling in Skin

    • Amy T. Ku, Qi Miao, Hoang Nguyen
    Pages 127-140

  16. The Generation of Organoids for Studying Wnt Signaling

    • Jarno Drost, Benedetta Artegiani, Hans Clevers
    Pages 141-159

  17. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells

    • Ian J. Huggins, David Brafman, Karl Willert
    Pages 161-181

  18. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions

    • Xiaoping Bao, Xiaojun Lian, Sean P. Palecek
    Pages 183-196

  19. Erratum to: Delivery of the Porcupine Inhibitor WNT974 in Mice

    • Li-shu Zhang, Lawrence Lum
    Pages E1-E1

  20. Back Matter

    Pages 197-198
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Keywords

About this book

Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.

Editors and Affiliations

  • University of Texas Southwestern Medical Center, Dallas, USA

    Quinn Barrett

  • Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, USA

    Lawrence Lum

Bibliographic Information

  • Book Title: Wnt Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Quinn Barrett, Lawrence Lum

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6393-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6391-1Published: 03 September 2016

  • Softcover ISBN: 978-1-4939-8185-4Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6393-5Published: 02 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 198

  • Number of Illustrations: 13 b/w illustrations, 32 illustrations in colour

  • Topics: Cell Biology

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