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Methods in Molecular Biology

Drosophila

Methods and Protocols

Editors: Dahmann, Christian (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-6371-3
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Hardcover $139.00
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Softcover $139.00
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About this book

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Using FlyBase, a Database of Drosophila Genes and Genomes

    Pages 1-31

    Marygold, Steven J. (et al.)

  • The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression

    Pages 33-52

    Caygill, Elizabeth E. (et al.)

  • The Q-System: A Versatile Expression System for Drosophila

    Pages 53-78

    Riabinina, Olena (et al.)

  • Analysis of MicroRNA Function in Drosophila

    Pages 79-94

    Chawla, Geetanjali (et al.)

  • Methods for High-Throughput RNAi Screening in Drosophila Cells

    Pages 95-116

    Billmann, Maximilian (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-6371-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-6369-0
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8179-3
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Drosophila
Book Subtitle
Methods and Protocols
Editors
  • Christian Dahmann
Series Title
Methods in Molecular Biology
Series Volume
1478
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6371-3
DOI
10.1007/978-1-4939-6371-3
Hardcover ISBN
978-1-4939-6369-0
Softcover ISBN
978-1-4939-8179-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 355
Number of Illustrations
31 b/w illustrations, 41 illustrations in colour
Topics

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