Methods in Molecular Biology

SUMO

Methods and Protocols

Editors: Rodriguez, Manuel S. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-6358-4
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Hardcover $179.99
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Softcover $139.00
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About this book

This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls.

Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Concepts and Methodologies to Study Protein SUMOylation: An Overview

    Pages 3-22

    Matunis, Michael J. (et al.)

  • The Regulation of Chromatin by Dynamic SUMO Modifications

    Pages 23-38

    Wilson, Nicole R. (et al.)

  • Reconstitution of the Recombinant RanBP2 SUMO E3 Ligase Complex

    Pages 41-54

    Ritterhoff, Tobias (et al.)

  • Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria

    Pages 55-65

    Brockly, Frédérique (et al.)

  • A Fluorescent In Vitro Assay to Investigate Paralog-Specific SUMO Conjugation

    Pages 67-78

    Eisenhardt, Nathalie (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-6358-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
SUMO
Book Subtitle
Methods and Protocols
Editors
  • Manuel S. Rodriguez
Series Title
Methods in Molecular Biology
Series Volume
1475
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6358-4
DOI
10.1007/978-1-4939-6358-4
Hardcover ISBN
978-1-4939-6356-0
Softcover ISBN
978-1-4939-8175-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 306
Number of Illustrations
20 b/w illustrations, 34 illustrations in colour
Topics