Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1472)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (18 protocols)
-
Front Matter
-
Computational Tools for Design and Assembly of Synthetic DNA
-
Front Matter
-
-
DNA Synthesis, Assembly, and Cloning
-
Front Matter
-
-
Reducing Error in Synthetic DNA
-
Front Matter
-
About this book
This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.
Editors and Affiliations
-
Applied Research Laboratories, The University of Texas at Austin, AUSTIN, USA
Randall A. Hughes
Bibliographic Information
Book Title: Synthetic DNA
Book Subtitle: Methods and Protocols
Editors: Randall A. Hughes
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6343-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017
Hardcover ISBN: 978-1-4939-6341-6Published: 27 September 2016
Softcover ISBN: 978-1-4939-8170-0Published: 27 June 2018
eBook ISBN: 978-1-4939-6343-0Published: 26 September 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 248
Number of Illustrations: 14 b/w illustrations, 60 illustrations in colour
Topics: Human Genetics