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Methods in Molecular Biology

Synthetic DNA

Methods and Protocols

Editors: Hughes, Randall A. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6343-0
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Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-6341-6
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-8170-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology.  Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • A Guide to Using STITCHER for Overlapping Assembly PCR Applications

    Pages 3-11

    O’Halloran, Damien M.

  • Synthetic Gene Design Using Codon Optimization On-Line (COOL)

    Pages 13-34

    Yu, Kai (et al.)

  • Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering

    Pages 35-45

    Milligan, John N. (et al.)

  • Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli

    Pages 49-61

    Zhong, Chao (et al.)

  • SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Synthetic Protein Libraries for Directed Evolution

    Pages 63-78

    Currin, Andrew (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6343-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-6341-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-8170-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic DNA
Book Subtitle
Methods and Protocols
Editors
  • Randall A. Hughes
Series Title
Methods in Molecular Biology
Series Volume
1472
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-6343-0
DOI
10.1007/978-1-4939-6343-0
Hardcover ISBN
978-1-4939-6341-6
Softcover ISBN
978-1-4939-8170-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 248
Number of Illustrations
14 b/w illustrations, 60 illustrations in colour
Topics