- Includes cutting-edge methods and protocols for meiosis research
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
- Table of contents (19 chapters)
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Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
Pages 1-23
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Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe
Pages 25-49
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Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast
Pages 51-98
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Ribosome Profiling for the Analysis of Translation During Yeast Meiosis
Pages 99-122
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Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation
Pages 123-132
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Table of contents (19 chapters)
- Download Preface 1 PDF (93.9 KB)
- Download Sample pages 1 PDF (399.9 KB)
- Download Table of contents PDF (98.5 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Meiosis
- Editors
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- David T. Stuart
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1471
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-6340-9
- DOI
- 10.1007/978-1-4939-6340-9
- Hardcover ISBN
- 978-1-4939-6338-6
- Softcover ISBN
- 978-1-4939-8169-4
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- X, 337
- Number of Illustrations
- 14 b/w illustrations, 45 illustrations in colour
- Topics
