Methods in Molecular Biology

Chromosome and Genomic Engineering in Plants

Methods and Protocols

Editors: Murata, Minoru (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.

 

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences

    Pages 1-13

    Graham, Nathaniel (et al.)

  • Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase

    Pages 15-30

    Li, Ruyu (et al.)

  • Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking

    Pages 31-47

    Chen, Weiqiang (et al.)

  • Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants

    Pages 49-61

    Xuan, Yuan Hu (et al.)

  • One-Step Generation of Chromosomal Rearrangements in Rice

    Pages 63-76

    Murata, Minoru (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-4931-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-4929-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8163-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chromosome and Genomic Engineering in Plants
Book Subtitle
Methods and Protocols
Editors
  • Minoru Murata
Series Title
Methods in Molecular Biology
Series Volume
1469
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-4931-1
DOI
10.1007/978-1-4939-4931-1
Hardcover ISBN
978-1-4939-4929-8
Softcover ISBN
978-1-4939-8163-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 218
Number of Illustrations
24 b/w illustrations, 38 illustrations in colour
Topics