Methods in Molecular Biology

Capillary Electrophoresis of Proteins and Peptides

Methods and Protocols

Editors: Tran, Nguyet Thuy, Taverna, Myriam (Eds.)

  • Includes cutting-edge methods and protocols for the study of CE of proteins and peptides
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts 
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-4014-1
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4012-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

Table of contents (17 chapters)

  • Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)–Fluorescence Analysis of Proteins

    Potier, Isabelle (et al.)

    Pages 1-10

  • Discrimination of Glycoproteins from Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection Coupled with Post-column Derivatization

    Kaneta, Takashi

    Pages 11-23

  • Capillary Zone Electrophoresis–Mass Spectrometry of Intact Proteins

    Domínguez-Vega, Elena (et al.)

    Pages 25-41

  • Screening of Small Intact Proteins by Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)

    Neuberger, Sabine (et al.)

    Pages 43-56

  • Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol–Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins

    Mokaddem, Meriem (et al.)

    Pages 57-66

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-4014-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4012-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Capillary Electrophoresis of Proteins and Peptides
Book Subtitle
Methods and Protocols
Editors
  • Nguyet Thuy Tran
  • Myriam Taverna
Series Title
Methods in Molecular Biology
Series Volume
1466
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-4014-1
DOI
10.1007/978-1-4939-4014-1
Hardcover ISBN
978-1-4939-4012-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 234
Number of Illustrations and Tables
30 b/w illustrations, 42 illustrations in colour
Topics