Skip to main content
SpringerLink
Log in

Chronic Myeloid Leukemia

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Shaoguang Li

    1. Department of Medicine Division of Hematology/Oncology, University of Massachusetts Medical School, Worcester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Haojian Zhang

    1. Medical Research Institute, Wuhan University, Wuhan, China

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential fro reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1465)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Molecular Detection of BCR-ABL in Chronic Myeloid Leukemia

    • Ya-Zhen Qin, Xiao-Jun Huang
    Pages 1-15

  3. Induction of Chronic Myeloid Leukemia in Mice

    • Haojian Zhang, Shaoguang Li
    Pages 17-25

  4. CML Mouse Model Generated from Leukemia Stem Cells

    • Yiguo Hu
    Pages 27-35

  5. Immunological Analyses of Leukemia Stem Cells

    • Kazuhito Naka, Yoshihiro Takihara
    Pages 37-45

  6. Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and Propidium Iodide

    • Ngoc DeSouza, Megan Zhou, Yi Shan
    Pages 47-57

  7. Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia

    • Eva S. Weissenberger, Daniela S. Krause
    Pages 59-72

  8. The Culture Repopulation Ability (CRA) Assay and Incubation in Low Oxygen to Test Antileukemic Drugs on Imatinib-Resistant CML Stem-Like Cells

    • Giulia Cheloni, Michele Tanturli
    Pages 73-85

  9. Tumor Suppressor Analysis in CML

    • Oliver Herrmann, Mirle Schemionek
    Pages 87-94

  10. Detecting Autophagy and Autophagy Flux in Chronic Myeloid Leukemia Cells Using a Cyto-ID Fluorescence Spectrophotometric Assay

    • Sujuan Guo, Kevin J. Pridham, Zhi Sheng
    Pages 95-109

  11. DREAM: A Simple Method for DNA Methylation Profiling by High-throughput Sequencing

    • Jaroslav Jelinek, Jozef Madzo
    Pages 111-127

  12. ChIP-seq Analysis of Human Chronic Myeloid Leukemia Cells

    • Lars Anders, Zhaodong Li
    Pages 129-138

  13. Quantitative Proteomics Analysis of Leukemia Cells

    • Sebastian Halbach, Jörn Dengjel, Tilman Brummer
    Pages 139-148

  14. A Convenient Cell Culture Model for CML Acquired Resistance Through BCR-ABL Mutations

    • Zhiqiang Wang, WenYong Chen
    Pages 149-157

  15. High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML

    • Leyuan Ma, Justine Roderick, Michelle A. Kelliher, Michael R. Green
    Pages 159-173

  16. Biological Analysis of Human CML Stem Cells; Xenograft Model of Chronic Phase Human Chronic Myeloid Leukemia

    • Sheela A. Abraham
    Pages 175-185

  17. New Mouse Models to Investigate the Efficacy of Drug Combinations in Human Chronic Myeloid Leukemia

    • Hanyang Lin, Adrian Woolfson, Xiaoyan Jiang
    Pages 187-205

  18. miRNome Analysis of CML Cells

    • Yadong Yang, Nan Ding, Xunong Dong, Xiangdong Fang
    Pages 207-217

  19. An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells

    • Farah J. Nassar, Rabab El Eit, Rihab Nasr
    Pages 219-241

  20. Single-Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies

    • Jonathan J. Chen, Minsuk Kwak, Rong Fan
    Pages 243-254
Back to top

Keywords

About this book

This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Medicine Division of Hematology/Oncology, University of Massachusetts Medical School, Worcester, USA

    Shaoguang Li

  • Medical Research Institute, Wuhan University, Wuhan, China

    Haojian Zhang

Bibliographic Information

  • Book Title: Chronic Myeloid Leukemia

  • Book Subtitle: Methods and Protocols

  • Editors: Shaoguang Li, Haojian Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-4011-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-4009-7Published: 31 August 2016

  • Softcover ISBN: 978-1-4939-8152-6Published: 09 June 2018

  • eBook ISBN: 978-1-4939-4011-0Published: 31 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 257

  • Number of Illustrations: 20 b/w illustrations, 43 illustrations in colour

  • Topics: Cancer Research

Publish with us

Policies and ethics

Back to top

Search

Navigation