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  • © 2016

Cilia

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1454)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiv
  2. Methods for Studying Ciliary Import Mechanisms

    • Daisuke Takao, Kristen J. Verhey
    Pages 1-14
  3. Targeting of ASH Domain-Containing Proteins to the Centrosome

    • Patricia Verdier, Stine K. Morthorst, Lotte B. Pedersen
    Pages 15-33
  4. Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy

    • Rania Ghossoub, Louise Lindbæk, Anahi Molla-Herman, Alain Schmitt, Søren Tvorup Christensen, Alexandre Benmerah
    Pages 35-51
  5. Methods to Study Interactions Between Ciliogenesis and Autophagy

    • Birgit Hegner Satir, Olatz Pampliega
    Pages 53-67
  6. Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium

    • Megan Smith Valentine, Judith L. Van Houten
    Pages 149-168
  7. STED and STORM Superresolution Imaging of Primary Cilia

    • T. Tony Yang, Weng Man Chong, Jung-Chi Liao
    Pages 169-192
  8. CLEM Methods for Studying Primary Cilia

    • Frank P. Macaluso, Geoffrey S. Perumal, Johan Kolstrup, Peter Satir
    Pages 193-202
  9. Methods for Visualization of Neuronal Cilia

    • Tamara Caspary, Daniela Marazziti, Nicolas F. Berbari
    Pages 203-214
  10. Methods to Study Centrosomes and Cilia in Drosophila

    • Swadhin Chandra Jana, Susana Mendonça, Sascha Werner, Monica Bettencourt-Dias
    Pages 215-236
  11. Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas

    • Emily L. Hunter, Winfield S. Sale, Lea M. Alford
    Pages 237-243
  12. Planaria as a Model System for the Analysis of Ciliary Assembly and Motility

    • Stephen M. King, Ramila S. Patel-King
    Pages 245-254
  13. Back Matter

    Pages 255-259

About this book

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

 

Editors and Affiliations

  • Albert Einstein College of Medicine, Yeshiva University, Bronx, USA

    Peter Satir

  • Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Søren Tvorup Christensen

Bibliographic Information

  • Book Title: Cilia

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Satir, Søren Tvorup Christensen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3789-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3787-5Published: 12 August 2016

  • Softcover ISBN: 978-1-4939-8139-7Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3789-9Published: 11 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 259

  • Number of Illustrations: 15 b/w illustrations, 39 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access