Cilia
Methods and Protocols
Editors: Satir, Peter, Christensen, Søren Tvorup (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.
- Table of contents (16 chapters)
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Methods for Studying Ciliary Import Mechanisms
Pages 1-14
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Targeting of ASH Domain-Containing Proteins to the Centrosome
Pages 15-33
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Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy
Pages 35-51
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Methods to Study Interactions Between Ciliogenesis and Autophagy
Pages 53-67
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Recombinant Reconstitution and Purification of the IFT-B Core Complex from Chlamydomonas reinhardtii
Pages 69-82
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Cilia
- Book Subtitle
- Methods and Protocols
- Editors
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- Peter Satir
- Søren Tvorup Christensen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1454
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3789-9
- DOI
- 10.1007/978-1-4939-3789-9
- Hardcover ISBN
- 978-1-4939-3787-5
- Softcover ISBN
- 978-1-4939-8139-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 259
- Number of Illustrations
- 15 b/w illustrations, 39 illustrations in colour
- Topics
