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Zebrafish

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Koichi Kawakami

    1. Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

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  2. E. Elizabeth Patton

    1. Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  3. Michael Orger

    1. Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1451)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
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  2. Genetics and Genomics

    1. Front Matter

      Pages 1-1
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    2. Chemical Screening in Zebrafish

      • Colleen A. Brady, Andrew J. Rennekamp, Randall T. Peterson
      Pages 3-16

    3. TALEN-Mediated Mutagenesis and Genome Editing

      • Alvin C. H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker
      Pages 17-30

    4. Homology-Independent Integration of Plasmid DNA into the Zebrafish Genome

      • Thomas O. Auer, Filippo Del Bene
      Pages 31-51

    5. Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System

      • Satoshi Ota, Atsuo Kawahara
      Pages 53-63

    6. Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish

      • An Xiao, Bo Zhang
      Pages 65-79

    7. Testing of Cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish Using PhiC31 Integrase

      • Yavor Hadzhiev, Irene Miguel-Escalada, Darius Balciunas, Ferenc Müller
      Pages 81-91

    8. Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines

      • Hideyuki Tanabe, Masahide Seki, Mari Itoh, Ailani Deepak, Pradeep Lal, Terumi Horiuchi et al.
      Pages 93-106

    9. Construction of the Inbred Strain

      • Minori Shinya
      Pages 107-118

  3. Disease Models and Mechanism

    1. Front Matter

      Pages 119-119
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    2. Zebrafish as a Model for the Study of Solid Malignancies

      • Genevieve C. Kendall, James F. Amatruda
      Pages 121-142

    3. Melanoma Regression and Recurrence in Zebrafish

      • Sonia Wojciechowska, Zhiqiang Zeng, James A. Lister, Craig J. Ceol, E. Elizabeth Patton
      Pages 143-153

    4. Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model

      • Claudia Tulotta, Shuning He, Lanpeng Chen, Arwin Groenewoud, Wietske van der Ent, Annemarie H. Meijer et al.
      Pages 155-169

    5. Modeling Leukemogenesis in the Zebrafish Using Genetic and Xenograft Models

      • Vinothkumar Rajan, Graham Dellaire, Jason N. Berman
      Pages 171-189

    6. Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos

      • Virginie Esain, Mauricio Cortes, Trista E. North
      Pages 191-206

    7. Live Imaging of Host–Pathogen Interactions in Zebrafish Larvae

      • Molly A. Matty, Stefan H. Oehlers, David M. Tobin
      Pages 207-223

    8. Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish

      • Jinhu Wang, Kenneth D. Poss
      Pages 225-235

    9. Studying Lipid Metabolism and Transport During Zebrafish Development

      • Erin M. Zeituni, Steven A. Farber
      Pages 237-255

  4. Neuroscience

    1. Front Matter

      Pages 257-257
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Keywords

About this book

This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish,  applications of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection and host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuits and their role in generating behavior. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful complement to the first book for new and experienced zebrafish researcher alike. 

Editors and Affiliations

  • Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

    Koichi Kawakami

  • Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

    E. Elizabeth Patton

  • Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

    Michael Orger

Bibliographic Information

  • Book Title: Zebrafish

  • Book Subtitle: Methods and Protocols

  • Editors: Koichi Kawakami, E. Elizabeth Patton, Michael Orger

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3771-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3769-1Published: 27 July 2016

  • Softcover ISBN: 978-1-4939-8134-2Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3771-4Published: 27 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 373

  • Number of Illustrations: 15 b/w illustrations, 64 illustrations in colour

  • Topics: Biochemistry, general

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