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Neurotransmitter Transporters

Investigative Methods

  • Book
  • © 2016

Overview

Editors:
  1. Heinz Bönisch

    1. Biomedical Center Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany

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  2. Harald H. Sitte

    1. Center of Physiology and Pharmacology Institute of Pharmacology, Medical University of Vienna, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 118)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Classical Radioligand Uptake and Binding Methods in Transporter Research: An Emphasis on the Monoamine Neurotransmitter Transporters

    • Sonja Sucic, Heinz Bönisch
    Pages 1-21

  3. Tracer Flux Measurements to Study Outward Transport by Monoamine Neurotransmitter Transporters

    • Thomas Steinkellner, Felix P. Mayer, Tina Hofmaier, Marion Holy, Therese Montgomery, Birgit Eisenrauch et al.
    Pages 23-40

  4. Interrogating the Activity of Ligands at Monoamine Transporters in Rat Brain Synaptosomes

    • John S. Partilla, Michael H. Baumann, Ann M. Decker, Bruce E. Blough, Richard B. Rothman
    Pages 41-52

  5. Using High-Speed Chronoamperometry to Measure Biogenic Amine Release and Uptake In Vivo

    • Lynette C. Daws, W. Anthony Owens, Glenn M. Toney
    Pages 53-81

  6. Experimental Data Guided Docking of Small Molecules into Homology Models of Neurotransmitter Transporters

    • Andreas Jurik, Amir Seddik, Gerhard F. Ecker
    Pages 83-89

  7. Immunohistochemical Methods for the Study of the Expression of Low-Affinity Monoamine Transporters in the Brain

    • Franck Louis, Thomas CouroussĂ©, Sophie Gautron
    Pages 91-108

  8. Analysis of Neurotransmitter Transporter Posttranslational Modifications: Phosphorylation and Palmitoylation

    • James D. Foster, Danielle E. Rastedt, Sathyavathi ChallaSivaKanaka, Roxanne A. Vaughan
    Pages 109-128

  9. Ensemble and Single Quantum Dot Fluorescence Methods in Neurotransmitter Transporter Research

    • Oleg Kovtun, Sandra J. Rosenthal
    Pages 129-141

  10. Approaches for Investigating Transporter Endocytic Trafficking in Cell Lines and Native Preparations

    • Haley E. Melikian, Sijia Wu, Luke R. Gabriel
    Pages 143-171

  11. Organic Cation Transport Measurements Using Fluorescence Techniques

    • Giuliano Ciarimboli, Eberhard Schlatter
    Pages 173-187

  12. Animal Models for Depression and the Mode of Action of Neurotransmitter Transporter-Blocking Antidepressants

    • Deeba Khan, Marianne Ronovsky, Thomas Steinkellner, Michael Freissmuth, Harald H. Sitte, Daniela D. Pollak
    Pages 189-202

  13. In Vivo Imaging of Dopamine Metabolism and Dopamine Transporter Function in the Human Brain

    • Matthäus Willeit, Ana Popovic, Lucie Bartova, Ulrich Sauerzopf, Martin Bauer, Nicole Praschak-Rieder
    Pages 203-220

  14. Back Matter

    Pages 221-229
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Keywords

About this book

The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Biomedical Center Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany

    Heinz Bönisch

  • Center of Physiology and Pharmacology Institute of Pharmacology, Medical University of Vienna, Vienna, Austria

    Harald H. Sitte

Bibliographic Information

  • Book Title: Neurotransmitter Transporters

  • Book Subtitle: Investigative Methods

  • Editors: Heinz Bönisch, Harald H. Sitte

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-3765-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3763-9Published: 20 August 2016

  • Softcover ISBN: 978-1-4939-8132-8Published: 21 April 2018

  • eBook ISBN: 978-1-4939-3765-3Published: 19 August 2016

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 229

  • Number of Illustrations: 23 b/w illustrations, 25 illustrations in colour

  • Topics: Neurosciences

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