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In Vivo Fluorescence Imaging

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mingfeng Bai

    1. Univ of Pittsburgh, Pittsburgh, USA

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  • Includes cutting-edge methods and protocols for in vivo fluorescence imaging
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1444)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Before In Vivo Imaging: Evaluation of Fluorescent Probes Using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays

    • Shaojuan Zhang
    Pages 1-13

  3. Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes

    • Xiaoxi Ling
    Pages 15-25

  4. In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models

    • Jie Cao, Mingzhou Zhou
    Pages 27-35

  5. FRET Imaging of Enzymatic Activities Using Smart Probes

    • Jinbo Li, Yan Zhang, Zhen Cheng
    Pages 37-43

  6. Fluorescence Imaging of Inflammation in Live Animals

    • Mingzhou Zhou, Jie Cao, Walter J. Akers
    Pages 45-54

  7. Fluorescence Lifetime Imaging of Cancer In Vivo

    • Owen Peng, Walter J. Akers
    Pages 55-66

  8. Noninvasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Tomography

    • Weizhou Hou, Steve H. Thorne
    Pages 67-72

  9. In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

    • Rui Huang, Peter S. Conti, Kai Chen
    Pages 73-84

  10. Intraoperative Fluorescence Imaging and Multimodal Surgical Navigation Using Goggle System

    • Christopher A. Mela, Francis A. Papay, Yang Liu
    Pages 85-95

  11. Utilization of Near-Infrared Fluorescent Imaging for Pharmaceutically Relevant Applications

    • Jelena M. Janjic, Sravan Kumar Patel, Christina Bagia
    Pages 97-108

  12. In Vivo Cell Tracking Using Two-Photon Microscopy

    • Daniela Malide
    Pages 109-122

  13. Small-Animal Imaging Using Diffuse Fluorescence Tomography

    • Scott C. Davis, Kenneth M. Tichauer
    Pages 123-137

  14. In Vivo Metal Ion Imaging Using Fluorescent Sensors

    • Genevieve C. Van de Bittner, Tasuku Hirayama
    Pages 139-152

  15. Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo

    • Qiaoya Lin, Huang Huang, Juan Chen, Gang Zheng
    Pages 153-166

  16. In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes

    • Guosong Hong, Hongjie Dai
    Pages 167-181

  17. Ex Vivo Imaging, Biodistribution, and Histological Study in Addition to In Vivo Imaging

    • Shaojuan Zhang
    Pages 183-191

  18. Experimental Design and Data Analysis of In Vivo Fluorescence Imaging Studies

    • Ying Ding, Hui-Min Lin
    Pages 193-210

  19. Back Matter

    Pages 211-213
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Keywords

About this book

This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking, image-guided treatment, and fluorescence imaging in the second NIR window, as well as protocols for evaluation methods before and after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous fields who wish to become morefamiliar with in vivo fluorescence imaging techniques.

Editors and Affiliations

  • Univ of Pittsburgh, Pittsburgh, USA

    Mingfeng Bai

Bibliographic Information

  • Book Title: In Vivo Fluorescence Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Mingfeng Bai

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3721-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3719-6Published: 10 June 2016

  • Softcover ISBN: 978-1-4939-8119-9Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3721-9Published: 09 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 213

  • Number of Illustrations: 8 b/w illustrations, 59 illustrations in colour

  • Topics: Imaging / Radiology

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