Methods in Molecular Biology

Non-Viral Gene Delivery Vectors

Methods and Protocols

Editors: Candiani, Gabriele (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Softcover $119.00
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About this book

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

Table of contents (18 chapters)

  • Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications

    Misra, Santosh K. (et al.)

    Pages 3-17

  • Cationic Lipid-Based Nucleic Acid Vectors

    Jubeli, Emile (et al.)

    Pages 19-32

  • Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles

    Falsini, Sara (et al.)

    Pages 33-43

  • Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA

    Barrán-Berdón, Ana L. (et al.)

    Pages 45-61

  • Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes

    Ojeda, Edilberto (et al.)

    Pages 63-75

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3718-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-3716-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-8118-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Non-Viral Gene Delivery Vectors
Book Subtitle
Methods and Protocols
Editors
  • Gabriele Candiani
Series Title
Methods in Molecular Biology
Series Volume
1445
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3718-9
DOI
10.1007/978-1-4939-3718-9
Hardcover ISBN
978-1-4939-3716-5
Softcover ISBN
978-1-4939-8118-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 290
Number of Illustrations
30 b/w illustrations, 36 illustrations in colour
Topics