Methods in Molecular Biology

Natural Killer Cells

Methods and Protocols

Editors: Somanchi, Srinivas S. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

 

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Tracking Effector and Memory NK Cells During MCMV Infection

    Beaulieu, Aimee M. (et al.)

    Pages 1-12

  • Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells

    Kay, Alexander W. (et al.)

    Pages 13-26

  • Assessment of NK Cell Metabolism

    Keppel, Molly P. (et al.)

    Pages 27-42

  • Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK Cells

    Erbe, Amy K., Ph.D. (et al.)

    Pages 43-56

  • Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH) and Flow Cytometry

    Maiti, Sourindra N., Ph.D.

    Pages 57-63

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3684-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3682-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-8116-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Natural Killer Cells
Book Subtitle
Methods and Protocols
Editors
  • Srinivas S. Somanchi
Series Title
Methods in Molecular Biology
Series Volume
1441
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3684-7
DOI
10.1007/978-1-4939-3684-7
Hardcover ISBN
978-1-4939-3682-3
Softcover ISBN
978-1-4939-8116-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 365
Number of Illustrations
27 b/w illustrations, 49 illustrations in colour
Topics