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Methods in Molecular Biology

Bacterial Cell Wall Homeostasis

Methods and Protocols

Editors: Hong, Hee-Jeon (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3676-2
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Hardcover $169.99
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Softcover $119.99
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  • ISBN 978-1-4939-8114-4
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About this book

This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.

 

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Atomic Force Microscopy Analysis of Bacterial Cell Wall Peptidoglycan Architecture

    Pages 3-9

    Turner, Robert D. (et al.)

  • Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure

    Pages 11-27

    Alvarez, Laura (et al.)

  • Microarray Analysis to Monitor Bacterial Cell Wall Homeostasis

    Pages 31-46

    Hong, Hee-Jeon (et al.)

  • Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes

    Pages 47-55

    Solis, Nestor (et al.)

  • Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli

    Pages 57-70

    Martorana, Alessandra M. (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3676-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3674-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-8114-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Cell Wall Homeostasis
Book Subtitle
Methods and Protocols
Editors
  • Hee-Jeon Hong
Series Title
Methods in Molecular Biology
Series Volume
1440
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3676-2
DOI
10.1007/978-1-4939-3676-2
Hardcover ISBN
978-1-4939-3674-8
Softcover ISBN
978-1-4939-8114-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 288
Number of Illustrations
26 b/w illustrations, 35 illustrations in colour
Topics