Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1440)
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Table of contents (19 protocols)
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Front Matter
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Analyzing Physical Properties of the Bacterial Cell Wall
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Front Matter
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Genome-Wide Approaches for the Identification of Gene Products with Roles in Cell Wall Homeostasis
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Front Matter
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Functional Analysis of Cell-Wall Associated Proteins
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Front Matter
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Reporter Assays for Cell Wall Stress
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Front Matter
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Analysis of the Non-Protein Components of the Cell Wall
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Front Matter
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Keywords
About this book
This volume brings
together the most widely used and important protocols currently being employed
in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial
Cell Wall Homeostasis cover a variety of subjects, such as: modern
microscopy techniques and other biophysical methods used to characterize the
subcellular structure of the bacterial cell wall; high-throughput approaches
that can be used to identify all the genes and proteins that participate in the
correct functioning of an organism’s cell wall; protocols for assaying
individual gene products for specific cell wall functions or identify chemicals
with inhibitory activity against the cell wall; and methods for analyzing the non-protein
components of the cell wall and the increasing use of computational approaches
for predicting and modeling cell wall related functions and processes. Written
in the highly successful Methods in Molecular Biology series format,
chapters include introduction to their respective topics, lists of the
necessary material and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols
emphasizes the diversity of the research taking place in bacterial cell wall
homeostasis, and explains how the integration of information from across
multiple disciplines is going to be essential if a holistic understanding of
this important process is to be obtained.
Editors and Affiliations
Bibliographic Information
Book Title: Bacterial Cell Wall Homeostasis
Book Subtitle: Methods and Protocols
Editors: Hee-Jeon Hong
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3676-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3674-8Published: 16 June 2016
Softcover ISBN: 978-1-4939-8114-4Published: 30 May 2018
eBook ISBN: 978-1-4939-3676-2Published: 16 June 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 288
Number of Illustrations: 26 b/w illustrations, 35 illustrations in colour
Topics: Microbiology