Bacterial Cell Wall Homeostasis
Methods and Protocols
Editors: Hong, Hee-Jeon (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.
- Table of contents (19 chapters)
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Atomic Force Microscopy Analysis of Bacterial Cell Wall Peptidoglycan Architecture
Pages 3-9
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Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure
Pages 11-27
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Microarray Analysis to Monitor Bacterial Cell Wall Homeostasis
Pages 31-46
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Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes
Pages 47-55
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Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli
Pages 57-70
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Table of contents (19 chapters)
- Download Preface 1 PDF (57.9 KB)
- Download Sample pages 1 PDF (494.3 KB)
- Download Table of contents PDF (83.7 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Bacterial Cell Wall Homeostasis
- Book Subtitle
- Methods and Protocols
- Editors
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- Hee-Jeon Hong
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1440
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3676-2
- DOI
- 10.1007/978-1-4939-3676-2
- Hardcover ISBN
- 978-1-4939-3674-8
- Softcover ISBN
- 978-1-4939-8114-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 288
- Number of Illustrations
- 26 b/w illustrations, 35 illustrations in colour
- Topics
