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Methods in Molecular Biology

High Throughput Screening

Methods and Protocols

Editors: Janzen, William P. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3673-1
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Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-3671-7
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-8113-7
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About this book

This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening,  high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Design and Implementation of High-Throughput Screening Assays

    Pages 1-32

    Powell, David J. (et al.)

  • Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method

    Pages 33-45

    Riera, Thomas V. (et al.)

  • High-Throughput Screening Using Mass Spectrometry within Drug Discovery

    Pages 47-63

    Rohman, Mattias (et al.)

  • Structure-Based Virtual Screening of Commercially Available Compound Libraries

    Pages 65-76

    Kireev, Dmitri

  • AlphaScreen-Based Assays: Ultra-High-Throughput Screening for Small-Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions

    Pages 77-98

    Yasgar, Adam (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3673-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-3671-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-8113-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
High Throughput Screening
Book Subtitle
Methods and Protocols
Editors
  • William P. Janzen
Series Title
Methods in Molecular Biology
Series Volume
1439
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3673-1
DOI
10.1007/978-1-4939-3673-1
Hardcover ISBN
978-1-4939-3671-7
Softcover ISBN
978-1-4939-8113-7
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XI, 308
Number of Illustrations
30 b/w illustrations, 71 illustrations in colour
Topics