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High Throughput Screening

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. William P. Janzen

    1. Epizyme, Inc., Cambridge, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1439)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Design and Implementation of High-Throughput Screening Assays

    • David J. Powell, Robert P. Hertzberg, Ricardo MacarrÏŒn
    Pages 1-32

  3. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method

    • Thomas V. Riera, Tim J. Wigle, Robert A. Copeland
    Pages 33-45

  4. High-Throughput Screening Using Mass Spectrometry within Drug Discovery

    • Mattias Rohman, Jonathan Wingfield
    Pages 47-63

  5. Structure-Based Virtual Screening of Commercially Available Compound Libraries

    • Dmitri Kireev
    Pages 65-76

  6. AlphaScreen-Based Assays: Ultra-High-Throughput Screening for Small-Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions

    • Adam Yasgar, Ajit Jadhav, Anton Simeonov, Nathan P. Coussens
    Pages 77-98

  7. Instrument Quality Control

    • Chatura Jayakody, Emily A. Hull-Ryde
    Pages 99-113

  8. Application of Fluorescence Polarization in HTS Assays

    • Xinyi Huang, Ann Aulabaugh
    Pages 115-130

  9. Time-Resolved Fluorescence Assays

    • Chen-Ting Ma, Eduard A. Sergienko
    Pages 131-142

  10. Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays

    • Peter Drueckes
    Pages 143-157

  11. Screening for Inhibitors of Kinase Autophosphorylation

    • Bianca Heedmann, Martin Klumpp
    Pages 159-169

  12. A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus

    • Thomas M. Smith, Daryl L. Richie, Jianshi Tao
    Pages 171-179

  13. Mycobacterium tuberculosis High-Throughput Screening

    • E. Lucile White, Nichole A. Tower, Lynn Rasmussen
    Pages 181-195

  14. Identification of State-Dependent Blockers for Voltage-Gated Calcium Channels Using a FLIPR-Based Assay

    • Alberto di Silvio, JeanFrancois Rolland, Michela Stucchi
    Pages 197-206

  15. A Luciferase Reporter Gene System for High-Throughput Screening of γ-Globin Gene Activators

    • Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li et al.
    Pages 207-226

  16. A High-Throughput Flow Cytometry Assay for Identification of Inhibitors of 3′,5′-Cyclic Adenosine Monophosphate Efflux

    • Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, Alexandre Chigaev
    Pages 227-244

  17. High-Throughput Cell Toxicity Assays

    • David Murray, Lisa McWilliams, Mark Wigglesworth
    Pages 245-262

  18. BRET: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells

    • Xiu-Lei Mo, Haian Fu
    Pages 263-271

  19. Application of Imaging-Based Assays in Microplate Formats for High-Content Screening

    • Adam I. Fogel, Scott E. Martin, Samuel A. Hasson
    Pages 273-304

  20. Back Matter

    Pages 305-308
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Keywords

About this book

This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening,  high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research.

Editors and Affiliations

  • Epizyme, Inc., Cambridge, USA

    William P. Janzen

Bibliographic Information

  • Book Title: High Throughput Screening

  • Book Subtitle: Methods and Protocols

  • Editors: William P. Janzen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3673-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3671-7Published: 18 June 2016

  • Softcover ISBN: 978-1-4939-8113-7Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3673-1Published: 17 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 308

  • Number of Illustrations: 30 b/w illustrations, 71 illustrations in colour

  • Topics: Biotechnology

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