Methods in Molecular Biology

Histone Deacetylases

Methods and Protocols

Editors: Sarkar, Sibaji (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.

Reviews

“This volume of Methods in Molecular Biology, Histone deacetylases, provides comprehensive overview of the methods and procedures for analysis of histone modifications, namely histone deacetylation. … Methodological tools required for more detailed determination of the molecular and biological roles of distinct HDACs and HDAC-containing complexes are summarized in this book, which will no doubt hasten these important mechanistic studies.” (Bozena Smolkova, Neoplasma, Vol. 64 (3), 2017)


Table of contents (22 chapters)

Table of contents (22 chapters)
  • A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity

    Pages 3-13

    Wu, Mei-Yi (et al.)

  • Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells

    Pages 15-22

    Citro, Simona (et al.)

  • Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial–Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3)

    Pages 23-29

    Wang, Jian-Qiu (et al.)

  • Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4)

    Pages 31-45

    Li, Lin (et al.)

  • Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5)

    Pages 47-84

    Guise, Amanda J. (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-3667-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $119.00
price for USA in USD
Softcover $119.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Histone Deacetylases
Book Subtitle
Methods and Protocols
Editors
  • Sibaji Sarkar
Series Title
Methods in Molecular Biology
Series Volume
1436
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3667-0
DOI
10.1007/978-1-4939-3667-0
Hardcover ISBN
978-1-4939-3665-6
Softcover ISBN
978-1-4939-8111-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 337
Number of Illustrations
21 b/w illustrations, 31 illustrations in colour
Topics