Methods in Molecular Biology

Synthetic mRNA

Production, Introduction Into Cells, and Physiological Consequences

Editors: Rhoads, Robert E. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3625-0
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Hardcover $169.99
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About this book

This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences
is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology. 

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences

    Pages 3-27

    Rhoads, Robert E.

  • Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experiments

    Pages 31-43

    Kowalska, Joanna (et al.)

  • Enzymatic Modification of 5′-Capped RNA and Subsequent Labeling by Click Chemistry

    Pages 45-60

    Holstein, Josephin M. (et al.)

  • Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG

    Pages 61-75

    Domashevskiy, Artem V. (et al.)

  • Intronless β-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements

    Pages 77-92

    Brown, Jessica A. (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3625-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3623-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-8098-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic mRNA
Book Subtitle
Production, Introduction Into Cells, and Physiological Consequences
Editors
  • Robert E. Rhoads
Series Title
Methods in Molecular Biology
Series Volume
1428
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-3625-0
DOI
10.1007/978-1-4939-3625-0
Hardcover ISBN
978-1-4939-3623-6
Softcover ISBN
978-1-4939-8098-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 320
Number of Illustrations
23 b/w illustrations, 39 illustrations in colour
Topics