Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1428)
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Table of contents (21 protocols)
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Front Matter
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Introduction
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Front Matter
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Synthesis of mRNA
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Front Matter
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Introduction of Synthetic mRNA into Cells
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Front Matter
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Alteration of Cellular Function with Synthetic mRNA
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Front Matter
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About this book
This volume
presents detailed laboratory protocols for in
vitro synthesis of mRNA with favorable properties, its introduction into
cells by a variety of techniques, and the measurement of physiological and
clinical consequences such as protein replacement and cancer immunotherapy. Synthetic
techniques are described for structural features in mRNA that provide investigational
tools such as fluorescence emission, click chemistry, photo-chemical
crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced
activation of the innate immune response. Protocols are described for clinical
applications such as large-scale transfection of dendritic cells, production of
GMP-grade mRNA, redirecting T cell specificity, and use of molecular
adjuvants for RNA vaccines. Written
in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessarymaterials and reagents, step-by-step
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Synthetic
mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource
for both laboratory investigators and clinicians interested in this powerful
and rapidly evolving technology.
Editors and Affiliations
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Dept of Biochemistry&Molecular Bio, Louisiana State Univ Health Scien center, Shreveport, USA
Robert E. Rhoads
Bibliographic Information
Book Title: Synthetic mRNA
Book Subtitle: Production, Introduction Into Cells, and Physiological Consequences
Editors: Robert E. Rhoads
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3625-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016
Hardcover ISBN: 978-1-4939-3623-6Published: 29 May 2016
Softcover ISBN: 978-1-4939-8098-7Published: 26 May 2018
eBook ISBN: 978-1-4939-3625-0Published: 28 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 320
Number of Illustrations: 23 b/w illustrations, 39 illustrations in colour
Topics: Human Genetics