Plant Cytogenetics
Methods and Protocols
Editors: Kianian, Shahryar F., Kianian, Penny M. A. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics. - About the authors
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Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108
Email: Shahryar.Kianian@ars.usda.gov
Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108
Email:P.Kianian@gmail.com
- Table of contents (15 chapters)
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C-Banding of Plant Chromosomes
Pages 1-5
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Chromosome Painting by GISH and Multicolor FISH
Pages 7-21
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Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes
Pages 23-33
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Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes
Pages 35-48
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Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Pages 49-64
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Table of contents (15 chapters)
- Download Preface 1 PDF (56.7 KB)
- Download Sample pages 1 PDF (224.4 KB)
- Download Table of contents PDF (93 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Plant Cytogenetics
- Book Subtitle
- Methods and Protocols
- Editors
-
- Shahryar F. Kianian
- Penny M. A. Kianian
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1429
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3622-9
- DOI
- 10.1007/978-1-4939-3622-9
- Hardcover ISBN
- 978-1-4939-3620-5
- Softcover ISBN
- 978-1-4939-8097-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 198
- Number of Illustrations
- 8 b/w illustrations, 32 illustrations in colour
- Topics
