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  • © 2016

RNA-Protein Complexes and Interactions

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for studying RNA-protein interactions
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1421)

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Softcover Book USD 119.99
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Hardcover Book USD 109.99
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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
  2. Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting

    • Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Ha Na Jang, Haihong Shen
    Pages 35-44
  3. Measuring mRNA Translation by Polysome Profiling

    • Marek Kudla, Fedor V. Karginov
    Pages 127-135

About this book

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand.  After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, RNA-Protein Complexes and Interactions: Methods and Protocols provides a set of useful protocols, both basic and advanced, designed to inspire researchers working with RNA and RNA-interacting proteins.

Editors and Affiliations

  • Beckman res.Ins.Dept.of Mol. & Cell Bio., Irell&Manella.Grad.Schl.Biolog.Sci., Duarte, USA

    Ren-Jang Lin

Bibliographic Information

  • Book Title: RNA-Protein Complexes and Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Ren-Jang Lin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3591-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3589-5Published: 11 March 2016

  • Softcover ISBN: 978-1-4939-8087-1Published: 19 April 2018

  • eBook ISBN: 978-1-4939-3591-8Published: 10 March 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 288

  • Number of Illustrations: 25 b/w illustrations, 24 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access