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Methods in Molecular Biology

RNA-Protein Complexes and Interactions

Methods and Protocols

Editors: Lin, Ren-Jang (Ed.)

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  • Includes cutting-edge methods and protocols for studying RNA-protein interactions
  • Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-3591-8
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3589-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8087-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand.  After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, RNA-Protein Complexes and Interactions: Methods and Protocols provides a set of useful protocols, both basic and advanced, designed to inspire researchers working with RNA and RNA-interacting proteins.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Characterization of RNA–Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2

    Pages 1-13

    Skrdlant, Lindsey (et al.)

  • Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)

    Pages 15-22

    Yoon, Je-Hyun (et al.)

  • Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro

    Pages 23-34

    Hou, Shuai (et al.)

  • Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting

    Pages 35-44

    Zheng, Xuexiu (et al.)

  • Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture

    Pages 45-52

    Kataoka, Naoyuki

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-3591-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3589-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8087-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
RNA-Protein Complexes and Interactions
Book Subtitle
Methods and Protocols
Editors
  • Ren-Jang Lin
Series Title
Methods in Molecular Biology
Series Volume
1421
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3591-8
DOI
10.1007/978-1-4939-3591-8
Hardcover ISBN
978-1-4939-3589-5
Softcover ISBN
978-1-4939-8087-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 288
Number of Illustrations
25 b/w illustrations, 24 illustrations in colour
Topics