Methods in Molecular Biology

Programmed Cell Death

Methods and Protocols

Editors: Puthalakath, Hamsa, Hawkins, Christine (Eds.)

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  • Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume contains protocols specifically designed for studying programmed cell death, and also discusses recent advances in techniques that span broader areas of biology that have been recently used or that have potential to be incorporated into cell death research. The protocols are mostly described in the context of mammalian systems, but also cover other systems such as plants, Drosophila, and yeast. Programmed Cell Death: Methods and Protocols is comprised of 20 chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9 describe methods for studying apoptosis associated with various pathologies in different organs including the lymphoid compartment, intestinal epithelium, granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques for studying apoptosis in non-mammalian systems; Chapters 14-16 cover biochemical and biophysical methods for studying Bcl-2 family protein dynamics and protein-protein interactions during apoptosis; and the last four chapters explore protocols that are useful not only in apoptosis research but in wider areas of biological research, such as genome editing, inducible transgenes, and proteomics. Written in the highly successful Methods in Molecular Biology aeries format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Programmed Cell Death: Methods and Protocols is a comprehensive and valuable resource for researchers, ranging from beginner to expert, in their studies on programmed cell death.

 

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry

    Sica, Valentina (et al.)

    Pages 1-16

  • In Vivo Apoptosis Imaging Using Site-Specifically 68Ga-Labeled Annexin V

    Bauwens, Matthias

    Pages 17-26

  • Detection of Active Caspases During Apoptosis Using Fluorescent Activity-Based Probes

    Edgington-Mitchell, Laura E. (et al.)

    Pages 27-39

  • Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation

    Parsons, Melissa J. (et al.)

    Pages 41-56

  • In Vitro Use of Peptide Based Substrates and Inhibitors of Apoptotic Caspases

    McStay, Gavin P.

    Pages 57-67

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3581-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3579-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8084-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Programmed Cell Death
Book Subtitle
Methods and Protocols
Editors
  • Hamsa Puthalakath
  • Christine Hawkins
Series Title
Methods in Molecular Biology
Series Volume
1419
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3581-9
DOI
10.1007/978-1-4939-3581-9
Hardcover ISBN
978-1-4939-3579-6
Softcover ISBN
978-1-4939-8084-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 291
Number of Illustrations
20 b/w illustrations, 39 illustrations in colour
Topics