Methods in Molecular Biology

The Mitotic Spindle

Methods and Protocols

Editors: Chang, Paul, Ohi, Ryoma (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3542-0
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About this book

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.

 

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Using Fluorescence Microscopy to Study Mitosis

    Pages 3-14

    Balchand, Sai K. (et al.)

  • Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation

    Pages 15-31

    He, Bin (et al.)

  • Purification and Fluorescent Labeling of Tubulin from Xenopus laevis Egg Extracts

    Pages 35-45

    Groen, Aaron C. (et al.)

  • Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro

    Pages 47-61

    Zanic, Marija

  • Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles

    Pages 63-75

    Lecland, Nicolas (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-3542-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $199.99
price for USA in USD
Softcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
The Mitotic Spindle
Book Subtitle
Methods and Protocols
Editors
  • Paul Chang
  • Ryoma Ohi
Series Title
Methods in Molecular Biology
Series Volume
1413
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3542-0
DOI
10.1007/978-1-4939-3542-0
Hardcover ISBN
978-1-4939-3540-6
Softcover ISBN
978-1-4939-8072-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 424
Number of Illustrations
27 b/w illustrations, 52 illustrations in colour
Topics